|
KMID : 0370220220660050236
|
|
Yakhak Hoeji
2022 Volume.66 No. 5 p.236 ~ p.241
|
|
|
Development and Validation of LC-MS/MS Method for Determination of Astaxanthin in Dietary Supplement
|
|
Park Ji-Eun
Oh Mi-Hyune
|
|
|
Abstract
|
|
|
|
The Ministry of Food and Drug Safety is amending its test methods for dietary supplements (functional healthfood) to establish regulatory standards and specifications in Korea. In this regard, we continue to research analyticalmethod development for the items to improve their sensitivity and selectivity. In this study, we have developed liquidchromatographic-tandem mass spectrometry for astaxanthin analysis. This method can be used to characterize and quantifydietary supplement formulations containing astaxanthin. The procedure was validated at 37.5-2,400 ng/mL. Standardcalibration curves presented linearity with the coefficient of determination (r2)>0.999. The limits of detection and limits ofquantitation were 16 and 49 ng/mL, respectively. The recovery results ranged between 91.9-97.4% at three differentconcentration levels with relative standard deviations (RSDs) of less than 3.6%. The precision results (RSDs) wereobtained between 1.2-3.5%, and reproducibility (1.8%) was obtained. These are in accordance with the establishedvalidation criteria (Association of Official Analytical Chemists). Our research can provide scientific evidence to amend theastaxanthin test method for Health Functional Food Codes.
|
|
|
KEYWORD
|
|
|
Astaxanthin, LC-MS/MS, Dietary supplement
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|